Fig. 2
From: Serine synthesis and catabolism in starved lung cancer and primary bronchial epithelial cells
Expression of serine synthesis pathway and one-carbon metabolism genes under serine/glycine or glucose deprivation. Normal BEC or cancer cells were treated with media containing different concentrations of glucose in combination with high/low levels of serine (150 µM/15 µM) and glycine (300 µM/30 µM) for 48 h. A Expression of SSP genes and B serine and glycine catabolic genes was assessed by quantitative PCR. Results are mean ± SEM from four (A549 and H1299) or three (BEC) experiments. Group comparisons were performed by Two-way ANOVA with Tukey post-hoc analysis; *P < 0.05; **P < 0.01; ***P < 0.001; n.s., not significant; ser/gly, serine/glycine; PHGDH, phosphoglycerate dehydrogenase; PSAT1, phosphoserine aminotransferase; SHMT1, serine hydroxymethyltransferase cytoplasmic isoform; SHMT2, serine hydroxymethyltransferase mitochondrial isoform; MTHFD1, methylenetetrahydrofolate dehydrogenase cytoplasmic isoform; MTHFD2, methylenetetrahydrofolate dehydrogenase mitochondrial isoform