Fig. 1

Serine synthesis pathway and one-carbon metabolism enzymes are overexpressed in lung cancer compared to normal lung. A PHGDH expression in lung cancer cell lines treated with 10 mM (high, H) or 0.2 mM (low, L) glucose with or without dialyzed serum (dFBS). Beta-actin was used as a loading control. B Gene expression in lung adenocarcinoma (LUAD, n = 514) or lung squamous cell carcinoma (LUSC, n = 502) and non-involved lung (n = 59 and n = 51, respectively) obtained from the publicly available TCGA dataset via the XENA database (https://xenabrowser.net/). Group comparisons were performed by Mann–Whitney U tests. * P < 0.05; *** P < 0.001; n.s., not significant. C Overall survival in lung adenocarcinoma patients (n = 491) from the TCGA dataset expressing low or high levels of MTHFD1/2. Median values were used as a cut-off. Survival analysis was performed using Log-rank test