Fig. 7

Import of FAs into mitochondria negatively affects cell viability. a Cell viability of MIA PaCa-2 cells treated 72 h with DGAT1i, DGAT2i, and ACCi in BCAA-containing or deficient medium. Values are percent of respective control with no addition of pharmacological inhibitor. One-way ANOVA followed by Tukey’s multiple comparisons test. N > 3, n > 12. Criteria of significance: ***p < 0.001, ****p < 0.0001. b Cell viability of MIA PaCa-2 treated with DGAT1i, DGAT1i + ACCi and DGAT1i + carnitine in complete medium, no glutamine, no glucose, and BCAA-depleted cells (-BCAA). Values are percent of respective control with no addition of pharmacological inhibitor. One-way ANOVA followed by Tukey’s multiple comparisons test. N > 3, n > 20. Criteria of significance: ***p < 0.001, ****p < 0.0001