Fig. 6

Inhibition of ACC rescues mitochondrial FA import and diminishes LDs. a Loss of LDs after ACCi treatment shown by confocal microscopy in MIA PaCa-2 cells. b Protein levels of AMPK, and phosphorylated AMPK (P-AMPK, Phospho-AMPKα (Thr172)). c Flow cytometry of PaTu 8902 and MIA PaCa-2 cells stained with BODIPY and treated with ACCi (10 µM, o/n), AICAR (500 µM, o/n), and compound C (10 µM, o/n) compared to controls. One-way ANOVA followed by Tukey’s multiple comparisons test. N = 3. n ≥ 5. d C16-BODIPY staining after ACCi, AICAR, and Compound C treatment demonstrated by confocal microscopy in MIA PaCa-2 cells