Fig. 2

BCAA starvation induces metabolic remodeling. a Partial least squares discriminant analysis (PLS-DA) scores plot (left) depicting the stratification of samples based on the presence/absence of BCAAs in PC3 (t3). PLS-DA was performed in Workflow4metabolomics using TIC-normalized data. The loadings plot corresponding to PLS-DA (right) shows a negative correlation between BCAA/BCKA and TG species. b Volcano plots of -BCAA-treated cells compared to +BCAA treatment depicting several lipid and metabolic classes, i.e. BCAA/BCKA, short-chain carnitines (SC-CAR), BCAA-derived carnitines (BCAA-CAR), long-chain carnitines (LC-CAR), cholesteryl-esters (CE), diacylglycerols (DG), cholesterol, triglycerides (TG), ether-linked TG (TG-O), free fatty acids (FA), free amino acids (AA), and TCA-cycle metabolites (TCA). Cells were starved 48 h prior the experiment. P-values were calculated by Welch’s t-test using the matrixTests package in RStudio