Fig. 2

HIGD1A knockdown inhibits cell proliferation and promotes hypoxia-induced apoptosis in HCC cells. A Western blotting assay for total HIGD1A protein expression following HIGD1A-knockdown in L02, HepG2, Huh7, and MHCC97H cells. B Proliferation ability of L02, HepG2, Huh7, and MHCC97H cells infected with shCtrl or shHIGD1A lentivirus as measured by CCK8 assay at the indicated time points. C Protein levels of caspase3 and cleaved-caspase3 were measured in L02, HepG2, Huh7, and MHCC97H cells infected with shCtrl or shHIGD1A lentivirus. D Flow cytometry analysis of Annexin V/7-AAD double-stained L02, HepG2, Huh7, and MHCC97H cells infected with shCtrl or shHIGD1A under normoxic or hypoxic condition for 12 h. Left, representative flow cytometric plot. Right, proportions of apoptotic cells. Results shown are mean ± SEM. *P <0.05, **P <0.01, ***P <0.001