Fig. 1
HIGD1A is overexpressed in HCC and is induced by hypoxia or low-glucose condition. A HIGD1A and HIF-1α protein levels in 24 pairs of HCC and adjacent normal tissues were analyzed by western blotting. The red color indicated pairs with increased HIGD1A expression in cancerous tissue. The grey scales of protein were analyzed by Image-Pro Plus software and were normalized by β-actin. T, tumor; N, non-tumor. B HIGD1A and HIF-1α mRNA levels in 24 pairs of HCC and adjacent normal tissues were analyzed by qRT-PCR. C Protein expression of HIGD1A and DNMT1 in L02, HepG2, Huh7, and MHCC97H cells. D Expression of HIGD1A mRNA in L02, HepG2, Huh7, and MHCC97H cells. E Western blotting analysis of HIGD1A protein expression in different cells cultured under 20% O2 or low glucose or 1% O2 for 12 h. F qRT-PCR analysis of HIGD1A mRNA expression in different cells cultured under 20% O2 or low glucose or 1% O2 for 12 h. Results were shown as mean ± SEM. *P <0.05, **P <0.01, ***P <0.001