Fig. 5

RPS27 mediates HFD-induced PCa metastasis A DU145 cells were treated with NEFA (50 μM OA + 50 μM PA) or BSA for 24 h, followed by Western blotting to detect RPS27 expression and quantification of RPS27 levels using ImageJ (right panel); B Migration and invasion assay of NEFA or BSA incubated DU145 cells; C Number of cells undergoing transmembrane movement, data shown as means ± SEM for each group from three independent experiments. Left panel: migration, right panel: invasion; D Representative images of liver tissues from HFD mice inoculated with control or RPS27_sh DU145 cells; E Representative hematoxylin and eosin stained images of the liver tissues; F Number of metastatic tumor nodules per liver section/mouse, n = 4; *P < 0.05; **P < 0.01; ***P < 0.001