Fig. 3

RPS27 enhanced PCa cell motility. A Western blot of RPS27 in normal prostate epithelial cells RWPE1 and various prostate cancer cells (LNCaP, 22RV1, DU145, and PC3); B Western blotting was used to detect the efficiency of RPS27 overexpression in DU145 and PC3; C-D Migration and invasion assay of control (EV) and RPS27-overexpressing (RPS27 OE) cells; Bar: 100 μm; (C) Number of cells undergoing transmembrane movement, data shown as means ± SEM for each group from three independent experiments; (D) Representative images; E Western blotting to detect the efficiency of RPS27 gene knockdown in DU145 and PC3; F-G Migration and invasion assay of control (NC) and RPS27 knockdown (RPS27_sh) cells; Bar: 100 μm; (F) Number of cells undergoing transmembrane movement, data shown as means ± SEM for each group from three independent experiments; (G) Representative images. *P < 0.05; **P < 0.01; ***P < 0.001