Fig. 4

PDE6H deletion altered nucleotide abundances as well as intermediates of the TCA cycle and sugar metabolites. There was an accumulation of mostly nucleotide triphosphates and a depletion of monophosphate nucleotides in PDE6H KO HCT116 compared with WT HCT116, measured by LC–MS. Red asterisks mark the accumulation, and green shows the depletion of the respective nucleotides upon light transduction [20]. Results are presented in the order of fold change between the KO and WT HCT116. Heatmap represents log₂ of the fold change of metabolite levels from the average abundances of all samples. Compounds which altered in abundance more than 20% are presented; N = 5 tissue culture replicates per experimental group, Q < 0.05 (FDR-corrected p-value)