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Fig. 3 | Cancer & Metabolism

Fig. 3

From: Cone photoreceptor phosphodiesterase PDE6H inhibition regulates cancer cell growth and metabolism, replicating the dark retina response

Fig. 3

PDE6H depletion increased intracellular cGMP levels and lowered mTORC1 activity. a cGMP levels of PDE6H KO HCT116 compared to mock control following treatment with 10-μM sildenafil citrate for 30 min (n = 4), colony sizes of PDE6H KO and mock PDE6H after a 10-day incubation with or without sildenafil (n = 6), and cGMP levels (n = 4) and colony counts of HCT116, NCI-H23, and MDA-MB-436 cells following PDE6H knockdown (n = 6) were measured. b Immunoblot analysis of WT and PDE6H KO HCT116 following sildenafil treatment. c Clonogenic survivals of PDE6H KO and mock HCT116 cells were assessed upon arginine depletion and rapamycin treatment, N = 6. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. d Immunoblot analysis of HCT116, NCI-H23, and MDA-MB-436 upon PDE6H knockdown. Representative images of Western blots are presented in Fig. 2b and d, n = 3

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Cancer & Metabolism

ISSN: 2049-3002