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Fig. 2 | Cancer & Metabolism

Fig. 2

From: Cone photoreceptor phosphodiesterase PDE6H inhibition regulates cancer cell growth and metabolism, replicating the dark retina response

Fig. 2

PDE6H knockdown induced cell cycle arrest and cell death in HCT116, NCI-H23, and MDA-MB-436. a Flow cytometry analysis revealed the percentage of cells in each cell cycle stage relative to the total number of viable cells. N = 6 biological replicates. b mRNA levels of PDE6G, PDE6H, PKG genes, and cell cycle regulators were measured following PDE6H knockdown. c Immunoblotting revealed that the levels of cell cycle regulators p21 and cyclin E1 increased upon PDE6H depletion. P-RB-795 levels decreased in HCT116 and NCI-H23. N = 3 biological replicates, quantifications of bands are normalised to those of β-actin values. d Cleaved caspase 3 + cells increased upon PDE6H knockdown in all three cell lines. PDE6H knockdown lowered percentage of live HCT116, NCI-H23, and MDA-MB-436 cells, increasing those of late apoptotic/necrotic cells. PDE6H knockdown also increased percentage of early apoptotic NCI-H23 cells. N = 6 biological replicates; error bars represent standard deviation (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001)

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Cancer & Metabolism

ISSN: 2049-3002