Fig. 3
From: Magnetospirillum magneticum triggers apoptotic pathways in human breast cancer cells
Measurement of caspase activity and identification of apoptotic markers induced by AMB-1 exposure. A and B Caspase activity measured over 30 min. The curves display the impact on breast cancer cells caused by different concentrations of AMB-1 bacteria and DFO (A) and the relative positive and negative controls, STS and zVAD (B). C and D Relative caspase activity extrapolated from graphs (A, B) and normalized to the control condition (n = 4 biological replicates per condition, statistical significance was assessed with an ordinary one-way ANOVA test). E Western blot analysis of breast cancer cell lysates. MDA-MB-231 were treated for 24 h in a hypoxic environment with either AMB-1, DFO, STS, and zVAD. Actin was used as a loading control for the analysis of the levels of protein detected. Full images of the membranes can be found in the Supplementary file 2 (Fig. S12)